Anti-EMI1 (Early Mitotic Inhibitor-1), Monoclonal Antibody
| Product Name | Anti-EMI1 (Early Mitotic Inhibitor-1), Monoclonal Antibody |
|---|---|
| Description | It recognizes a 56kDa protein, which is identified as Early Mitotic Inhibitor-1 (EMI1). It regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Emi1 is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The Emi1 protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, Emi1 is transcriptionally induced by the E2F transcription factor. Emi1 overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of Emi1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry. |
| Synonyms | Early Mitotic Inhibitor-1 (EMI1), FBX5, Fbxo31 |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | EMI1/1176 |
| Immunogen | Recombinant fragment (203 amino acid residues between aa 1-250) of human EMI1 protein |
| Isotype | IgG2a, kappa |
| Reactivity | Human |
| Applications | FC, IF, IHC, WB |
| Form | 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 1mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
| Gene Id | 26271 |
| Uniprot | Q9UKT4 |
| Storage | Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required. |
| References | Reimann, J.D., et al. 2001. Emi1 is a mitotic regulator that interacts with Cdc20 and inhibits the anaphase promoting complex. Cell 105: 645-655. |
| Supplier | ARP |
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