| Product Name | Anti-ERK2 Antibody [PCRP-MAPK1-1D1] |
|---|---|
| Description | Mouse monoclonal [PCRP-MAPK1-1D1] antibody to ERK2. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | PCRP-MAPK1-1D1 |
| Conjugate | Unconjugated |
| Immunogen | Recombinant full-length human MAPK1 protein. |
| Isotype | IgG2b |
| Molecular Weight | 41.3 kDa |
| Target | ERK2 |
| Specificity | Mitogen-activated protein kinase (MAPK) signaling pathways involve two closely-related MAP kinases, known as extracellular-signal-related kinase 1 (ERK 1, p44) and 2 (ERK 2, p42). Growth factors, steroid hormones, G protein coupled receptor ligands and neurotransmitters can initiate MAPK signaling pathways. Activation of ERK 1 and ERK 2 requires phosphorylation by upstream kinases such as MAP kinase (MEK), MEK kinase and Raf-1. ERK 1 and ERK 2 phosphorylation can occur at specific tyrosine and threonine sites mapping within consensus motifs that include the threonine-glutamate-tyrosine motif. ERK activation leads to dimerization with other ERKs and subsequent localization to the nucleus. Active ERK dimers phosphorylate serine and threonine residues on nuclear proteins and influence a host of responses that include proliferation, differentiation, transcription regulation and development. |
| Reactivity | Human |
| Applications | ELISA, FC, IF, IP |
| Working Dilutions | IP: 1-2µg / 100-500µg proteins, Flow Cytometry: 0.5-2 µg/million cells, IF: 0.5-2 µg/ml |
| Form | Liquid |
| Diluent Buffer | Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide. |
| Concentration | 200 µg/ml |
| Storage | Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. |
| Supplier | Antibodies.com |
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