| Product Name | Anti-Fas Antibody [rB-R18] |
|---|---|
| Description | Recombinant mouse monoclonal [rB-R18] antibody to Fas. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | rB-R18 |
| Conjugate | Unconjugated |
| Immunogen | Recombinant human CD95 protein. |
| Isotype | IgG2a |
| Molecular Weight | 38-50 kDa |
| Target | Fas |
| Specificity | MAb rB-R18 specifically recognizes CD95, also known as Fas, a transmembrane glycoprotein with a MW of 40-45kDa, containing 8kDa of N-glycoside-linked polysaccharide. It is a receptor for TNFSF6/FASLG, a member of the nerve growth factor receptor/tumor necrosis factor superfamily, mediating receptor-triggered apoptosis. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation, which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). CD95 antigen is expressed on the surface of various cell types, preferentially on the CD45RAlow CD45ROhigh subset of memory T lymphocytes. |
| Reactivity | Human |
| Applications | IHC-Fr, FA, FC, IF |
| Working Dilutions | Functional Studies, Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml, IHC: Frozen: 0.5-1 µg/ml |
| Form | Liquid |
| Diluent Buffer | Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide. |
| Concentration | 200 µg/ml |
| Storage | Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. |
| Supplier | Antibodies.com |
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