Anti-Fibroblasts (CD90, Thy-1) (Hu) from Mouse (AS02) - FITC (100 tests)
| Product Name | Anti-Fibroblasts (CD90, Thy-1) (Hu) from Mouse (AS02) - FITC (100 tests) |
|---|---|
| Description |
The monoclonal antibody clone AS02 reacts specifically with human CD90 (Thy-1), a GPI-anchored glycoprotein of the immunoglobulin superfamily with a molecular weight of 25 – 35 kDa (3). CD90 (Thy-1) in human is primarily expressed by nerve cells, additionally in a sub-population (20%) of CD34+ blood stem cells and in various fibroblasts (1-8). In contrast to mouse and rat it is not expressed by thymocytes and peripheral blood T cells. AS02 recognizes fibroblasts of different origin but does not react with human blood cells, keratinocytes, resting micro-/macrovascular endothelial cells and components of the extracellular matrix like fibronectin, collagen type I, III, IV and laminin. Thus, AS02 is especially suitable for the specific detection and cell separation of human fibroblasts (2). Activated endothelial cells, after stimulation in vitro or in tissue sec- tions of inflamed tissue in vivo, bind AS02 with different intensities, implying that CD90 (Thy-1) can be considered to be an activation marker of human endothelial cells. Recent papers demonstrate the binding of AS02 with activated microvascular endothelial cells (4,5), with a subpopulation of lung fibroblasts (6), specialized lymph node fibroblasts, but not lymphocytes (7) and normal or inflamed synovial fibroblasts in human (8). In immunoblot analysis AS02 detects nonreduced CD90 at 30 kDa (1,3). Contamination with fibroblasts is common in primary cultures of various cell types. Fast-growing fibroblasts can rapidly overgrow the desired cells (e.g. endothelial cells, keratinocytes, adrenal cortex cells, thyrocytes) in vitro, making it difficult to draw any meaningful conclusions from the resulting mixed cultures. Thus, a rapid and efficient means to eliminate fibroblasts is essential for reproducible results with primary cell cultures. AS02 coupled to magnetic beads allows an effi- cient means to remove fibroblasts from mixed cultures. Clearly, the same method allows the isolation of small numbers of fibroblasts from such cultures for further analysis. |
| Host | Mouse |
| Clone | AS02 |
| Immunogen | Human skin fibroblasts |
| Isotype | IgG1, kappa |
| Specificity | Fibroblasts (CD90, Thy-1) |
| Reactivity | Human |
| Applications | FC |
| Form | FITC-conjugated Mouse IgG1 PBS with 2% BSA and 0,05% NaN3, pH 7,4. The antibody was purified from culture supernatant by mouse IgG-specific affinity chromatography. FITC was coupled using standard procedures and unbound FITC was eliminated by gel chromatography. |
| Storage | The antibody is stable for 9 months when stored as undiluted liquid at 2-8°C. |
| Notes | RUO |
| Supplier | Dianova GmbH |
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