| Product Name | Anti-GATA3 Antibody [1A12-1D9] |
|---|---|
| Description | Mouse monoclonal [1A12-1D9] antibody to GATA3. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 1A12-1D9 |
| Conjugate | Unconjugated |
| Immunogen | Purified partial recombinant recombinant human GATA3 protein expressed in E. coli. |
| Isotype | IgG1 |
| Molecular Weight | Approximately 55 kDa in SH-SY5Y cell lysate and SH-SY5Y nuclear extract. |
| Target | GATA3 |
| Specificity | This antibody recognises GATA3 also known as trans-acting T-cell-specific transcription factor GATA-3 or GATA-binding factor 3.The GATA3 protein is a member of the GATA family of transcription factors which has received its name from binding DNA sequences containing the GATA consensus motif 5'-[AT]GATA[AG]-3' (P15976). GATA3 has been shown to play a critical role in T cell development and the differentiation of T helper 2 (Th2) cells. In addition, GATA3 has been implied to have tissue specific functions such as a regulatory role in the development of the mammary gland epithelium and skin tissue. Aberrant GATA3 levels have also been identified in a variety of cancers including breast cancer. Indeed, GATA3 has become a marker for certain breast cancer types and GATA3 antibodies are commonly used for the immunohistochemical evaluation of breast cancer samples. |
| Reactivity | Human, Mouse, Rat |
| Applications | ICC, IF, IHC-P, IP, WB |
| Working Dilutions | ICC: 1:50 - 1:100, IF: 1:50 - 1:100, IHC-P: 1:200 - 1:2,000, IP: 5 ug, WB: 1:1,000 |
| Form | Liquid |
| Diluent Buffer | Supplied in Phosphate Buffered Saline with 1 mg/ml BSA, 30% Glycerol, and <0.1% Sodium Azide. |
| Concentration | 1 mg/ml |
| Storage | Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. |
| Supplier | Antibodies.com |
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