| Product Name | Anti-Gli3 Antibody |
|---|---|
| Description | Rabbit polyclonal antibody to Gli3. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | Synthetic peptide corresponding to amino acids 41-57 of human Gli3 protein. |
| Isotype | IgG |
| Target | Gli3 |
| Amino Acid Sequence | NEDESPGQTYHRERRNA |
| Protein Length | 17 |
| Specificity | This antibody recognises the human Zinc Finger protein 3 (GLI-3), also known as GLI-Kruppel family member 3, a 169.8 kDa protein belonging to the GLI C2H2-type zinc finger protein of transcription factors.GLI-3 and the related GLI-2 proteins are the primary transcription factors that mediate Sonic hedgehog (Shh) signals in the mouse, GLI-2 being primarily a transcriptional activator while GLI-3 is a transcriptional repressor. GLI-3 plays a role in the development of the limbs and brain. It is expressed in a wide range of adult tissues including the colon, lung, spleen, testis and placenta.Defects in GLI3 are causative of a range of disease states including Pallister-Hall syndrome, type IV preaxial polydactyly, acrocallosal syndrome (ACS) and Greig cephalo-poly-syndactyly syndrome.GLI proteins are also implicated as potent oncogenes in the embryonal carcinoma cell. |
| Reactivity | Amphibian, Gallus, Monkey, Aves, Canine, Human, Mouse, Primate, Rat |
| Applications | ELISA, IHC-P, WB |
| Working Dilutions | ELISA: 1:6,000, IHC-P: 1.25 µg/ml, WB: 1:500 |
| Form | Liquid |
| Diluent Buffer | Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide. |
| Concentration | 500 µg/ml |
| Storage | Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. |
| Supplier | Antibodies.com |
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