| Product Name | Anti-Glucocorticoid Receptor Antibody [5E4] |
|---|---|
| Description | Mouse monoclonal [5E4] antibody to Glucocorticoid Receptor. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 5E4 |
| Conjugate | Unconjugated |
| Immunogen | A peptide corresponding to amino acids 150-176 of human GCR linked to human thyroglobulin. |
| Isotype | IgG1 |
| Target | Glucocorticoid Receptor |
| Amino Acid Sequence | APTEKEFPKTHSDVSSEQQHLKGQTGT |
| Protein Length | 27 |
| Specificity | This antibody recognises the human glucocorticoid receptor, also known as Nuclear receptor subfamily 3 group C member 1 (NR3C1), a 777 amino acid receptor bearing 3 distinct functional domains, an N-terminal modulating domain, a DNA binding domain and a C-terminal steroid binding domain. The human glucocorticoid receptor is located either in the cytoplasm of cells, prior to ligand binding associated with certain heat shock proteins , or the nucleus following binding to ligand. Mutations in the glucocorticoid receptor gene can lead to familial glucocorticoid resistance, characterized by elevated plasma cortisol levels. Mouse anti human glucocorticoid receptor antibody, clone 5E4 has been demonstrated to cross-react with the murine glucocorticoid receptor |
| Reactivity | Human, Mouse |
| Applications | FC |
| Working Dilutions | FC 1: Neat - 1:10, Use 10µl of the suggested working dilution to label 1x106 cells in 100µl. |
| Form | Liquid |
| Diluent Buffer | Supplied in Phosphate Buffered Saline with 0.1% BSA and 0.1% Sodium Azide. |
| Concentration | 1 mg/ml |
| Storage | Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. |
| Supplier | Antibodies.com |
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