| Product Name | Anti-Glutamate Receptor 1 (AMPA subtype) Antibody |
|---|---|
| Description | Rabbit polyclonal antibody to Glutamate Receptor 1 (AMPA subtype). |
| Host | Rabbit |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | Synthetic peptide sequence SHSSGMPLGATGL corresponding to the C terminal region of the native molecule conjugated to Bovine Serum Albumin. |
| Isotype | IgG |
| Molecular Weight | Approximately 110 kDa co-migrating with GluR1 expressed in transfected cells. |
| Target | Glutamate Receptor 1 (AMPA subtype) |
| Amino Acid Sequence | SHSSGMPLGATGL |
| Specificity | This antibody recognises the glutamate receptor 1 (also known as AMPA) which acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.This antibody linked to immobilized Protein A immunoprecipitates detergent-solubilized receptor from brain or transfected cells. |
| Reactivity | Monkey, Hamster, Human, Rat |
| Applications | IHC-Fr, IP, WB |
| Working Dilutions | IHC-Fr: 1 µg/ml - 3 µg/ml, WB: 0.5 µg/ml - 2.5 µg/ml, Paraformaldehyde or paraformaldehyde/glutaraldehyde are suitable. Cryostat and vibratome sections can be used with or without Triton X-100 treatment. Western blot analysis of rat brain shows a single band of approximately 110 kDa co-migrating with GluR1 expressed in transfected cells. |
| Form | Liquid |
| Diluent Buffer | Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide. |
| Concentration | 1 mg/ml |
| Storage | Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. |
| Supplier | Antibodies.com |
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