Anti-Glycophorin C Cytoplasmic domain Monoclonal Antibody, PE
| Product Name | Anti-Glycophorin C Cytoplasmic domain Monoclonal Antibody, PE |
|---|---|
| Description | Mouse monoclonal antibody to human Glycophorin C (Cytoplasmic domain) PE conjugate. Glycophorin C (GPC, sialoglycoprotein beta, glycoconnectin) and glycophorin D (GPD, sialoglycoprotein s) are closely related erythrocyte transmembraneous glycoproteins, which are products of the same glycophorin C gene. The GPC (Mr 40K) amino acid sequence consists of 128 amino acids which traå_verses the bilayer once. GPC has three domains: (i) a glycosylated N-terminal extracellular domain (residues 1-57) containing about 12 O-glycans and 1 N-glycan; (ii) a hydrophobic intramembranous domain (58-81) which spans the lipid bi-layer; and (iii) a C-terminal cytoplasmic domain (82-128). GPD is identical to GPC but lacks the first 21 NH2-terminal amino acid residues2,. GPC is coded on chromosome 2q14-q2. The functional role of GPC and GPD is in the regulation of shape and membrane mechanical properå_ties of the red cell through the interaction of their cytoplasmic domains with membrane protein 4.1 within the cytoskeleton network. GPC carries the Gerbich blood group antigens. GPC is deficient in erythrocytes of the Leach type of Gerbich negative. The other types of Gerbich negative, Gerbich type and Yus type, have altered forms of GPC. Rare individuals of the Leach phenotype lacking GPC and GPD have elliptocytic red cells. GPC expression is not confined to erythroid cells3 as GPC has a fairly broad distribution in both erythroid and non-erythroid tissues4. Previously 50-100,000 copies of GPC per red cell were estimated. By the use of Fab fragments of monoclonal antibodies, the number of molecules per red cell has been estimated at 143,0005. |
| Synonyms | GPC, sialoglycoprotein beta, glycoconnectin |
| Clonality | Monoclonal |
| Clone | BGRL 100 |
| Immunogen | Synthetic peptide comprising of 17 amino acid residues within the cytoplaså_mic C-terminal domain. Glycophorin C has a fairly broad distribution in erythroid and non-erythroid tissues. |
| Isotype | Mouse IgG1 kappa light chain |
| Reactivity | Human |
| Applications | FC, IF |
| Form | PE-conjugate, in PBS buffer, pH 7.2 containing 0.1% sodium azide, 1% BSA |
| Storage | 2-8C, do not freeze |
| References | 1. Cartron, J.P., et al. (1990) In Blood Cell Biochemistry, Vol. 1: Erythroid Cells (ed. J.R. Harris) 299-335. Plenum Press 2. Tanner, M.J.A, et al. (1988(, Biochem J. 250, 407-414 3. Anstee, D.J. (1990) Vox Sang. 58: 1-20 (review) 4. Anstee, D.J., et al. (1992) In Biochemistry of the erythrocyte polypeptide blood group antigens, ed. Agre P & Cartron, J.P. John Hopkins University Press. 5. Smythe J et al (1994) Blood 83 6 1668-1672 6. Reid M.E. et al. (1990), Transf. Med. 1, Suppl. 1, p 66 (Abstract). 7. King et al., (1995) Brit. J. Haem., 89: 440-448 |
| Supplier | ARP |
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