| Product Name | Anti-GTPase HRAS Antibody [H-Ras-03] |
|---|---|
| Description | Mouse monoclonal [H-Ras-03] antibody to GTPase HRAS. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | H-Ras-03 |
| Conjugate | Unconjugated |
| Immunogen | Synthetic peptide sequence DIHQYREQIKRVKDSDDC of human H-Ras. |
| Isotype | IgG1 |
| Target | GTPase HRAS |
| Amino Acid Sequence | DIHQYREQIKRVKDSDDC |
| Specificity | This antibody recognises the human GTPase HRas also known as H-Ras, Transforming protein p21 or p21ras. H-Ras is a ubiquitously expressed lipid-anchored GTPase belonging to the Ras superfamily of small GTPases, which acts as a molecular signal transduction on/off switch on the inner surface of the plasma membrane and endomembranes. H-Ras, N-Ras and K-Ras are highly related RAS genes which regulate pathways involved in cell differentiation, proliferation, adhesion, migration and apoptosis and are known proto-oncogenes. H-Ras is located within the peripheral plasma membrane and within endomembrane compartments including the ER and Golgi apparatus. In the ER H-Ras can be activated by the RasGRF family exchange factors RasGRF1 and RasGRF2, this process does not appear to happen withing the Golgi. Mutations in the H-Ras gene are responsible for the rare condition known as Costello syndrome characterized by facial, cardiovascular and musculoskeletal abnormalities and mental retardation. Mouse anti Human H-Ras, clone H-Ras-03 has been used for the detection of H-Ras in the MCF10A human epitheliod breast cancer cell line by Western blotting. |
| Reactivity | Human, Mouse, Rat |
| Applications | ELISA, WB |
| Working Dilutions | WB: 0.5 - 1 µg/ml |
| Form | Liquid |
| Diluent Buffer | Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide. |
| Concentration | 1 mg/ml |
| Storage | Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. |
| Supplier | Antibodies.com |
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