| Product Name | Anti-Hamartin Antibody |
|---|---|
| Description | Rabbit polyclonal antibody to Hamartin. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | A peptide corresponding to 15 amino acids near the centre of human TSC1. |
| Isotype | IgG |
| Molecular Weight | Approximately 130 kDa in mouse EL4 cell lysates. |
| Target | Hamartin |
| Protein Length | 15 |
| Specificity | This antibody recognises an epitope within the internal region of the 130kDa tumour suppressor protein, Tumour sclerosis complex 1 (TSC1). Mutation within the TSC1 gene is responsible for Tuberous sclerosis (TSC), an autosomal dominant genetic disorder associated with the development of benign tumors (hamartomas), a condition also induced by mutation of the Tumour sclerosis complex 2 (TSC2) gene.Many proteins have been shown to play a role in the regulation of the serine/threonine kinase known as target of rapamycin (TOR), a central component in a complex signaling pathway which controls cell proliferation and cell cycle progression. Negative regulation of TOR activity occurs following the over-expression of the TSC1 and TSC2 gene products, hamartin and tuberin. These act by suppressing the phosphorylation of eukaryotic translation initiation factor 4E binding protein 1 (4EBP1) and ribosomal protein S6 kinase (S6K), downstream targets of TOR involved in mRNA translation. Furthermore the TOR activator protein Rheb has been identified as a target of TSC1-TSC2 complexes.Rabbit anti Human TSC1 antibody has been reported as suitable for use in IHC on mouse EL4 cell cytospins at 2µg/ml. |
| Reactivity | Human, Mouse, Rat |
| Applications | IHC-Fr, IHC-P, WB |
| Working Dilutions | WB: 1 µg/ml |
| Form | Liquid |
| Diluent Buffer | Supplied in Phosphate Buffered Saline with 0.02% Sodium Azide. |
| Concentration | 1 mg/ml |
| Storage | Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. |
| Supplier | Antibodies.com |
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