| Product Name | Anti-HDAC3 Antibody [PCRP-HDAC3-3C9] |
|---|---|
| Description | Mouse monoclonal [PCRP-HDAC3-3C9] antibody to HDAC3. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | PCRP-HDAC3-3C9 |
| Conjugate | Unconjugated |
| Immunogen | Recombinant full-length human HDAC3 protein. |
| Isotype | IgG2b |
| Molecular Weight | 49 kDa |
| Target | HDAC3 |
| Specificity | In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino-terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated RPD3) and HDAC3, all of which are related to the yeast transcriptional factor Rpd3p, have been identified as histone deacetylases. |
| Reactivity | Human |
| Applications | ELISA, FC, IF, IP, WB |
| Working Dilutions | WB: 1-2 µg/ml, IP: 1-2µg / 100-500µg proteins, Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml |
| Form | Liquid |
| Diluent Buffer | Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide. |
| Concentration | 200 µg/ml |
| Storage | Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. |
| Supplier | Antibodies.com |
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