Anti-HIF1 alpha (Hypoxia-Inducible Factor 1-alpha), Monoclonal Antibody
| Product Name | Anti-HIF1 alpha (Hypoxia-Inducible Factor 1-alpha), Monoclonal Antibody |
|---|---|
| Description | HIF1 (hypoxia-inducible factor 1), a heterodimeric transcription factor complex central to cellular response to hypoxia, consists of two subunits (HIF-1 alpha and HIF-1 beta) which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family. Expression of HIF-1 alpha protein is regulated by cellular oxygen level alterations as well as in oxygen-independent manner via different cytokines (through the PI3K-AKT-mTOR pathway), growth factors, oncogenic activation, or loss of tumor suppressor function etc. In normoxic cells, HIF-1 alpha is proline hydroxylated leading to a conformational change that promotes its binding to the VLH (von Hippel Lindau) protein E3 ligase complex; ubiquitination and followed by rapid proteasomal degradation. Hypoxia as well as chemical hydroxylase inhibitors (desferrioxamine, cobalt etc.) inhibit HIF-1 alpha degradation and lead to its accumulation in the cells, whereas, contrastingly, HIF-1 beta/ARNT (AhR nuclear translocator) remains stable under both conditions. Besides their critical role in hypoxic response, HIF1s regulates the transcription of genes responsible for angiogenesis, erythropoiesis/iron-metabolism, glucose metabolism, cell proliferation/survival, adipogenesis, carotid body formation, B lymphocyte development and immune reactions. |
| Synonyms | ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78 (bHLHe78), Hypoxia inducible factor 1 alpha, Member of PAS superfamily 1 (MOP1), PAS domain-containing protein 8 (PASD8) |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | HIF1A/84 |
| Immunogen | Recombinant human HIF1 alpha protein |
| Isotype | IgG2b, kappa |
| Reactivity | Human |
| Applications | FC, IF |
| Form | 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
| Gene Id | 3091 |
| Uniprot | Q16665 |
| Storage | Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required. |
| References | Wang GL and Semenza GL. 1993. Proc. Natl. Acad. Sci. 90:4304. 2. Wang GL and Semenza GL. 1993. J. Biol. Chem. 268:21513. 3. Wang GL and Semenza GL. 1995. J. Biol. Chem. 270:1230. 4. Galbraith MD, et al. 2013. Cell. 153:1327 |
| Supplier | ARP |
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