| Product Name | Anti-HLA B27 Antibody [HLA-ABC-m3] (FITC) |
|---|---|
| Description | Mouse monoclonal [HLA-ABC-m3] antibody to HLA B27 (FITC). |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | HLA-ABC-m3 |
| Conjugate | FITC |
| Immunogen | Immune complex precipitated from an HLA-B27 positive cell line by anti HLA antibody and staphylococcal protein A. |
| Isotype | IgG2a |
| Target | HLA B27 |
| Specificity | This antibody recognises the HLA-B27 alloantigen and reacts with the peripheral blood lymphocytes of 47/47 individuals conventionally typed as HLA-B27 + and precipitates cell surface molecules of 43 and 12 kDa, corresponding to the HLA Class 1 heavy chain and beta 2 microglobulin.Scatchard affinity analysis shows that Mouse anti Human HLA B27 antibody, clone HLA-ABC-m3 has a higher affinity for HLA-B27 (9.7 x 108M-1) than for HLA B7 (9.5 x 107 M-1).Flow cytometry - subjects expected fluorescence intensity:Heterozygous HLA-B27+ = Strong.Homozygous HLA-B27+ = Strong.Heterozygous HLA-B7+ = Faint.Non B27, Non B7 = Negative. |
| Reactivity | Human |
| Applications | FC |
| Working Dilutions | Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 106 cells or 100µl of whole blood. |
| Form | Liquid |
| Diluent Buffer | Supplied in Phosphate Buffered Saline with 0.2% BSA and 0.1% Sodium Azide. |
| Concentration | 50 µg/ml |
| Storage | Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. |
| Supplier | Antibodies.com |
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