| Product Name | Anti-HLA-DR Monoclonal Antibody |
|---|---|
| Description | HLA-DR, a member of MHC class II glycoproteins, that bind intracellularly processed peptides and present them to the Th cells, is composed of 36 kDa alpha chain and 27 kDa beta chain, both anchored in the plasma membrane. Together with other MHC II molecules HLA-DR plays a central role in the immune system. It is expressed on antigen-presenting cells (dendritic cells, B lymphocytes, monocytes, macrophages). The reagent is intended as isotype control for flow cytometry analysis to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this isotype control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 µg per ml of incubation mixture), change the conditions of your experiment to reduce the background. |
| Clone | L243 |
| Immunogen | Human B lymphocytes |
| Isotype | IgG2a |
| Specificity | The mouse monoclonal antibody L243 recognizes specifically HLA-DR molecules, both peptide-loaded and empty. |
| Reactivity | Canine, Human, Primate |
| Applications | FA, FC, ICC, IHC, IP, WB |
| Form | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide. |
| Storage | Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. |
| Background | HLA-DR, a member of MHC class II glycoproteins, that bind intracellularly processed peptides and present them to the Th cells, is composed of 36 kDa alpha chain and 27 kDa beta chain, both anchored in the plasma membrane. Together with other MHC II molecules HLA-DR plays a central role in the immune system. It is expressed on antigen-presenting cells (dendritic cells, B lymphocytes, monocytes, macrophages). |
| Supplier | Exbio |
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