| Product Name | Anti-HLA DRB Antibody [SPM289] |
|---|---|
| Description | Mouse monoclonal [SPM289] antibody to HLA DRB. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | SPM289 |
| Conjugate | Unconjugated |
| Immunogen | Activated human peripheral blood mononuclear cells. |
| Isotype | IgG2b |
| Molecular Weight | ~28 kDa (beta chain) |
| Target | HLA DRB |
| Specificity | This antibody reacts with the beta-chain of HLA-DR antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. The L243 antibody recognizes a different epitope than the SPM289 monoclonal antibody, and these antibodies do not cross-block binding to each others respective epitopes. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36kDa alpha (heavy) chain and a 28kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts. |
| Reactivity | Human |
| Applications | FC, IF, IHC-P, WB |
| Working Dilutions | Flow Cytometry: 1-2 µg/million cells, IF: 2-4 µg/ml, WB: 1-2 µg/ml, IHC-P: 1-2 µg/ml |
| Form | Liquid |
| Diluent Buffer | Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide. |
| Concentration | 200 µg/ml |
| Storage | Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. |
| Supplier | Antibodies.com |
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