| Product Name | Anti-Horse IgE Antibody [7H2] |
|---|---|
| Description | Mouse monoclonal [7H2] antibody to Horse IgE. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 7H2 |
| Conjugate | Unconjugated |
| Immunogen | Equine IgE. |
| Isotype | IgG1 |
| Molecular Weight | Approximately 200 kDa in non-reducing conditions. This clone is not suitable for use in Western Blotting under reducing conditions. For Western Blotting applications it is recommended to use clone 3H10 MCA5982GA. |
| Target | Horse IgE |
| Specificity | This antibody recognises native equine IgE and does not cross react with equine IgM, IgA or IgG.IgE is an immunoglobulin primarily produced from plasma cells and, in normal serum, present at very low concentrations. Western blot analysis against affinity purified equine IgE using Mouse anti Horse IgE clone 7H2 demonstrates a single major band of approximately 200 kDa under non-reducing conditions that corresponds with the expected molecular weight of the complete equine IgE molecule (Wilson, D.A. et al. 2006).IgE is important in both type 1 hypersensitivity and immunity to parasite infections, in particular parasitic worms where equine IgE levels are significantly elevated following worm infection.Monoclonal antibodies to equine IgE are of particular relevance to research into insect bite sensitivity, one of the most widely studied allergic diseases in equid species (Schaffartzik, A., et al. 2012). |
| Reactivity | Equine |
| Applications | IHC-Fr, ELISA, IHC-P, WB |
| Working Dilutions | ELISA: 1:40 - 1:400 |
| Form | Liquid |
| Diluent Buffer | Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide. |
| Concentration | 1 mg/ml |
| Storage | Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. |
| Supplier | Antibodies.com |
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