| Product Name | Anti-HSP27 (Heat Shock Protein 27), Monoclonal Antibody |
|---|---|
| Description | This monoclonal antibody reacts specifically with heat shock protein HSP27 in human and monkey tissues and cell lines such as MCF-7. HSP27, also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins produced by all organisms studied. HSP27 synthesis is induced by elevated temperature, as well as by estrogen in hormone responsive cells. Interestingly, human HSP27 also shares greater than 50% homology with low molecular weight Drosophila HSPs and mammalian α-crystalline lens protein. Because of the estrogen responsive nature of HSP27, this protein has been studied extensively in human estrogen responsive tissues such as cervix, endometrium and breast tissue. Therefore HSP27 may be useful in classifying various hormone sensitive tumors. |
| Synonyms | Heat shock 27kDa protein, Estrogen-regulated 24kDa protein, Heat shock 25kDa protein 1, Heat shock 28kDa protein 1, Heat shock protein beta-1, HMN2B, Hsp25, Hsp28, HspB1, Stress-responsive protein 27 (SRP27) |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | G3.1 |
| Immunogen | Partially purified hsp27 (earlier called 24K) protein from breast cancer MCF-7 cells. |
| Isotype | IgG1, kappa |
| Reactivity | Gallus, Human, Mouse, Primate, Rat, Sheep |
| Applications | FC, IF, IHC, WB |
| Form | 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml. |
| Gene Id | 3315 |
| Uniprot | P04792 |
| Storage | Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required. |
| References | Edwards DP et. al. Biochem Biophys Research Commun, 93:804-812, 1980.Ciocca DR et. al. Breast Cancer Research and Treatment, 20:33-42, 1991 |
| Supplier | ARP |
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