| Product Name | Anti-HUWE1 / Mule Antibody |
|---|---|
| Description | Rabbit polyclonal antibody to HUWE1 / Mule. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Immunogen | Synthetic peptide corresponding to 19 amino acids near the carboxy terminal of human HUWE1. |
| Isotype | IgG |
| Target | HUWE1 / Mule |
| Protein Length | 19 |
| Specificity | This antibody recognises human E3 ubiquitin-protein ligase HUWE1, also known as HUWE1, ARF-binding protein 1, ARF-BP1, HECT, UBA and WWE domain-containing protein 1, Homologous to E6AP carboxyl terminus homologous protein 9, HectH9, Large structure of UREB1, LASU1, Mcl-1 ubiquitin ligase E3, Mule or Upstream regulatory element-binding protein 1. HUWE1 is a 4374 amino acid DNA binding protein with a predicted molecular mass of ~480 kDa.HUWE1 is an E3 ubiquitin-protein ligase involved in mediating ubiquitination and subsequent proteasomal degradation of target proteins. HUWE1 is also involved in apoptosis through catalyzation of the poly-ubiquitination and degradation of MCL1, ubiquitination of the p53 tumor suppressor and core histones including H1, H2A, H2B, H3 and H4. Binds to an upstream initiator-like sequence in the preprodynorphin gene. Research indicates that HUWE1 regulates the abundance of CDC6 after DNA damage by polyubiquitinating and targeting CDC6 to degradation. |
| Reactivity | Human, Mouse, Rat |
| Applications | IHC-P, WB |
| Working Dilutions | IHC-P: 5 µg/ml, WB: 2 µg/ml, This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections. |
| Form | Liquid |
| Diluent Buffer | Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide. |
| Concentration | 1 mg/ml |
| Storage | Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. |
| Supplier | Antibodies.com |
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