| Product Name | Anti-iC3b Antibody [013III-1.16] |
|---|---|
| Description | Mouse monoclonal [013III-1.16] antibody to iC3b. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 013III-1.16 |
| Conjugate | Unconjugated |
| Immunogen | Purified human iC3b. |
| Isotype | IgG2b |
| Target | iC3b |
| Specificity | This antibody recognises the ~42 kDa human inactive complement component 3b (iC3b) neoantigen present in blood serum. Cleavage of C3b into iC3b by the protease factor I, in the presence of cofactors, changes the structure and resulting binding properties, preventing binding by complement factor B and properdin. This prevents further progression of the complement pathway, providing another mechanism by which the complement pathway may be regulated. iC3b is thought to play a role in inducing tolerance , binding complement receptor type 3 on antigen presenting cells and stimulating them to produce transforming growth factor beta2 and interleukin-10. It is thought to stimulate B-cells via the CD21 receptor providing a link between the innate and adaptive immune responses. Additionally iC3b acts as an opsonin which is recognised by complement receptor 3 expressed on leukocytes or complement receptor of the immunoglobulin superfamily (CRIg) on Kupffer cells, stimulating phagocytosis of the tagged cells. iC3b levels are often elevated in diseases such as Systemic Lupus Erythematosis and Rheumatoid arthritis.iC3b levels are often elevated in diseases such as Systemic Lupus Erythematosis and Rheumatoid arthritis. |
| Reactivity | Human |
| Applications | IHC-Fr, ELISA, FC, IHC-P, WB |
| Working Dilutions | IHC-P: 10 µg/ml |
| Form | Liquid |
| Diluent Buffer | Supplied in Borate Buffered Saline with <0.1% Sodium Azide. |
| Concentration | Lot Specific |
| Storage | Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. |
| Supplier | Antibodies.com |
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