| Product Name | Anti-IL-2 Receptor alpha Antibody [K231.3B2] |
|---|---|
| Description | Mouse monoclonal [K231.3B2] antibody to IL-2 Receptor alpha. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | K231.3B2 |
| Conjugate | Unconjugated |
| Immunogen | Con A activated porcine peripheral blood lymphocytes. |
| Isotype | IgG1 |
| Target | IL-2 Receptor alpha |
| Specificity | This antibody recognises porcine CD25, the alpha chain of the interleukin 2 receptor (IL-2Ra), also known as the low affinity Interleukin 2 receptor. The IL-2 receptor exists in three forms, the high affinity heterodimer, the intermediate affinity ß monomer and the low affinity a monomer configurations. Clone K231.3B2 was clustered as CD25 at the First International Workshop to Define Swine Cluster of Differentiation (CD) Antigens. Mouse anti pig CD25, clone K231.3B2 immunoprecipitates a protein of ~65-70 kDa from activated lymphocyte preparations. CD25 is a 270 amino acid single pass type I transmembrane glycoprotein containing 2 Sushi domains. Low expression of CD25 is seen on resting peripheral blood mononuclear cells, rapidly up-regulated following stimulation by concanavalin A and phorbol myristate acetate, indicative of its role as an activation antigen. |
| Reactivity | Porcine |
| Applications | IHC-Fr, FC |
| Working Dilutions | Flow Cytometry: 1:25 - 1:200, Use 10µl of the suggested working dilution to label 1x106 cells in 100µl |
| Form | Liquid |
| Diluent Buffer | Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide. |
| Concentration | 1 mg/ml |
| Storage | Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. |
| Supplier | Antibodies.com |
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