| Product Name | Anti-Integrin alpha 4 / CD49D Antibody [Bu49] (PE) |
|---|---|
| Description | Mouse monoclonal [Bu49] antibody to Integrin alpha 4 / CD49D (PE). |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | Bu49 |
| Conjugate | PE |
| Immunogen | Hyatt myeloma cell line. |
| Isotype | IgG1 |
| Target | Integrin alpha 4 / CD49D |
| Specificity | This antibody recognises human CD49d, also known as integrin alpha 4 or VLA-4 alpha subunit. CD49d is a ~150 kDa single pass type I transmembrane glycoprotein. CD49d can be proteolytically cleaved to yield fragments of ~80 and ~70 kDa. CD49d associates with either CD29 to form VLA-4 or with Integrin beta-7 to form The Peyer's patches-specific homing receptor LPAM-1, involved in the lymphocyte migration and homing to gut-associated lymphoid tissue (Sackstein 2006) through its interaction with MadCam-1, preferentially expressed on Peyer's patch high endothelial venules and postcapillary venules in lamina propria. CD49d is expressed on a broad range of cells including lymphocytes, monocytes, eosinophils and thymocytes. CD49d expression may serve as a prognostic parker of survival and disease progression in patients with chronic lymphocytic leukemia and may have therapeutic potential. Mouse anti human CD49, clone Bu49 cross-reacts with the canine B-cell leukemia cell line GL-1 and induces homotypic cell aggregation. |
| Reactivity | Canine, Human |
| Applications | FC |
| Working Dilutions | Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 1x106 cells in 100µl |
| Form | Lyophilized |
| Diluent Buffer | Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide. |
| Concentration | Reconstitution dependent. |
| Storage | Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. |
| Supplier | Antibodies.com |
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