| Product Name | Anti-Integrin alpha 4 / CD49D Antibody [PS/2] (PE) |
|---|---|
| Description | Rat monoclonal [PS/2] antibody to Integrin alpha 4 / CD49D (PE). |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | PS/2 |
| Conjugate | PE |
| Immunogen | P815 DBA/2 murine mastocytoma cells. |
| Isotype | IgG2b |
| Target | Integrin alpha 4 / CD49D |
| Specificity | This antibody recognises mouse alpha 4 integrin (CD49d), a ~150 kDa single pass type I membrane glycoprotein that can associate with either beta 1 integrin (CD29) or beta 7 integrin to form heterodimers CD49d/CD29 (VLA-4) and alpha4/beta7 (LPAM-1) respectively. CD49d is expressed on most lymphocytes, granulocytes, monocytes and thymocytes. The primary ligands for CD49d are CD106 (VCAM-1), fibronectin and MAdCAM-1. Clone PS/2 has also been reported to block the binding of CD49d to its ligands. |
| Reactivity | Human, Mouse |
| Applications | FC |
| Working Dilutions | Flow Cytometry: Neat - 1:10, Use 10µl of the suggested working dilution to label 106 cells in 100µl. The Fc region of monoclonal antibodies may bind to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/BUF041B). |
| Form | Lyophilized |
| Diluent Buffer | Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide. |
| Concentration | Reconstitution dependent. |
| Storage | Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. |
| Supplier | Antibodies.com |
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