| Product Name | Anti-Integrin beta 1 Antibody [12G10] (PE) |
|---|---|
| Description | Mouse monoclonal [12G10] antibody to Integrin beta 1 (PE). |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 12G10 |
| Conjugate | PE |
| Immunogen | Purified human beta1 integrin preparation from HT1080 fibrosarcoma cell extract. |
| Isotype | IgG1 |
| Target | Integrin beta 1 |
| Specificity | This antibody recognises human CD29 also known as beta1 integrin or VLA-4 subunit alpha. CD29 is a ~130 kDa under reducing, ~115 kDa under non-reducing conditions single pass type I transmembrane glycoprotein. CD29 acts as the common beta subunit of the heterodimeric very late antigens 1-6, complexing with CD49a-f respectively where it forms part of the receptors for laminin , collagen and fibronectin. the VLA heterodimers mediate cell-cell and cell-matrix interactions. Mouse anti Human CD29, clone 12G10 binding to cells adhering via VLA-4 results in actin cytoskeletal disruption and subsequent inhibition of attachment and spreading whilst 12G10 binding to cells adhering via VLA-5 results in enhancement of both these processes. Clone 12G10 enhances alpha 5 beta 1-fibronectin interactions and binds to a region of CD25 containing the binding epitopes of several other anti CD29 antibody clones. However, unlike these, binding of 12G10 is enhanced in the presence of ligands such as fibronectin fragments. Binding of antibody clone 12G10 to the integrin ß1 subunit is affected by divalent cations and the binding epitope appears to be located around residues 207-218 in the b1 subunit putative A-domain. |
| Reactivity | Human, Mink, Rabbit |
| Applications | FC |
| Working Dilutions | Flow Cytometry: Neat |
| Form | Lyophilized |
| Diluent Buffer | Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide. |
| Concentration | Reconstitution dependent. |
| Storage | Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. |
| Supplier | Antibodies.com |
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