| Product Name | Anti-Ki-67 Monoclonal Antibody |
|---|---|
| Description | Ki-67 is a highly protease-sensitive nuclear protein expressed in two isoforms (345 kDa and 395 kDa), both of which are identified by the antibody clone Ki-67. The Ki-67 antigen is essential for cell proliferation and its expression is restricted to the cycling cells. It is detected in G1, S, G2 and M phase, whereas it is absent in cells which are in G0 phase and it is not associated with DNA repair processes. Ki-67 thus represents an important tool for detection of proliferating cells, which is of great importance in tumor diagnostics and is commonly used as a prognostic factor in cancer studies. The reagent is designed for immunofluorescence analysis.Suggested working dilution for flow cytometry is 8 µg/ml. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator. |
| Clone | Ki-67 |
| Immunogen | Nuclei of the Hodgkin lymphoma cell line L428 |
| Isotype | IgG1 |
| Specificity | The mouse monoclonal antibody Ki-67 recognizes Ki-67 antigen, a non-histone nuclear protein expressed exclusively in proliferating cells. |
| Reactivity | Bovine, Human |
| Applications | FC, ICC, IHC, WB |
| Form | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide. |
| Storage | Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. |
| Background | Ki-67 is a highly protease-sensitive nuclear protein expressed in two isoforms (345 kDa and 395 kDa), both of which are identified by the antibody clone Ki-67. The Ki-67 antigen is essential for cell proliferation and its expression is restricted to the cycling cells. It is detected in G1, S, G2 and M phase, whereas it is absent in cells which are in G0 phase and it is not associated with DNA repair processes. Ki-67 thus represents an important tool for detection of proliferating cells, which is of great importance in tumor diagnostics and is commonly used as a prognostic factor in cancer studies. |
| Supplier | Exbio |
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