| Product Name | Anti-LAG-3 Antibody [C9B7W] (PE) |
|---|---|
| Description | Rat monoclonal [C9B7W] antibody to LAG-3 (PE). |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | C9B7W |
| Conjugate | PE |
| Immunogen | Murine CD223 Ig fusion protein. |
| Isotype | IgG1 |
| Target | LAG-3 |
| Specificity | This antibody recognises mouse lymphocyte activation gene-3 (LAG-3), a ~70 kDa activation-induced cell surface molecule that is also referred to as CD223. Murine CD223 is expressed on activated CD4 positive and CD8 positive alpha/beta T lymphocytes and a subset of natural killer (NK) cells. CD223 binds to MHC class II molecules with high affinity and is reported to negatively regulate T cell homeostasis and T cell expansion. Clone C9B7W recognises an epitope within the D2 domain of CD223. Rat anti Mouse CD223 antibody, clone C9B7W is reported to block the in vitro function of murine LAG-3 but does not block binding of LAG-3 to MHC class II. |
| Reactivity | Mouse |
| Applications | FC |
| Working Dilutions | Flow Cytometry: Neat - 1:5, Use 10µl of the suggested working dilution to label 106 cells in 100µl. The Fc region of monoclonal antibodies may bind to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/BUF041B). |
| Form | Lyophilized |
| Diluent Buffer | Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide. |
| Concentration | Reconstitution dependent. |
| Storage | Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. |
| Supplier | Antibodies.com |
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