| Product Name | Anti-Lck Monoclonal Antibody |
|---|---|
| Description | Lck is a Src-family tyrosine kinase, which is essential for signaling through the T-cell receptor (TCR) complex. Upon TCR triggering, Lck phosphorylates the ITAM motives in its zeta subunits, establishing binding sites for the SH2 domains of the tyrosine kinase ZAP70, which is also phosphorylated by Lck and thereby activated to generate subsequent signaling platforms by phosphorylation of adaptor LAT. Whereas the majority of Lck is localized to the plasma membrane, there is also a significant fraction associated with the Golgi apparatus, which may contribute to Raf activation under conditions of weak stimulation through the TCR. Lck is also involved in the regulation of apoptosis induced by various stimuli, but not by the death receptors. The reagent is designed for Flow Cytometry analysis of human blood cells using 5 μl reagent / 100 μl of whole blood or 106 cells in a suspension.The content of a vial (0.5 ml) is sufficient for 100 tests. |
| Clone | LCK-01 |
| Immunogen | Peptide corresponding to amino acids 22-36 in the sequence of human Lck. |
| Isotype | IgG1 |
| Specificity | The antibody LCK-01 recognizes defined epitope (aa 22-36) of Lck, a 56 kDa Src-family protein tyrosine kinase. |
| Reactivity | Human |
| Applications | FC, ICC, IP, WB |
| Form | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
| Storage | Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. |
| Background | Lck is a Src-family tyrosine kinase, which is essential for signaling through the T-cell receptor (TCR) complex. Upon TCR triggering, Lck phosphorylates the ITAM motives in its zeta subunits, establishing binding sites for the SH2 domains of the tyrosine kinase ZAP70, which is also phosphorylated by Lck and thereby activated to generate subsequent signaling platforms by phosphorylation of adaptor LAT. Whereas the majority of Lck is localized to the plasma membrane, there is also a significant fraction associated with the Golgi apparatus, which may contribute to Raf activation under conditions of weak stimulation through the TCR. Lck is also involved in the regulation of apoptosis induced by various stimuli, but not by the death receptors. |
| Supplier | Exbio |
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