| Product Name | Anti-Ly-6B.2 Alloantigen Antibody [7/4] |
|---|---|
| Description | Rat monoclonal [7/4] antibody to Ly-6B.2 Alloantigen. |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | 7/4 |
| Conjugate | Unconjugated |
| Immunogen | Cultured bone marrow cells. |
| Isotype | IgG2a |
| Target | Ly-6B.2 Alloantigen |
| Specificity | This antibody recognises the Ly-6B.2 antigen. Ly-6B.2 is a ~25-30 kDa GPI-anchored, heavily glycosylated protein expressed on neutrophils, inflammatory monocytes and some activated macrophages. High levels of expression are seen in bone marrow, spleen, lung and lymph nodes. N-glycanase treatment of thioglycollate elicited peritoneal neutrophil lysates lowers the apparent molecular weight of Ly-6B.2 to ~15 kDa. In common with other Ly-6 antigens Ly-6B.2 demonstrates a polymorphic expression on inbred mouse strains. recognises the Ly-6B.2 antigen in 129J; AKR; C57BL/6; C57BL/10; C58; DBA/2; NZB; NZW; SJL; MFI; Swiss (PO) Strains whilst A2G; A/Sn; ASW; BALB/c; C3H/HEH: CBA.T6T6 are negative or demonstrate very weak reactivity. Rat anti mouse Ly-6B.2 has been successfully used for the immunomagnetic depletion of neutrophils during the enrichment of primitive hematopoietic cells from bone marrow and the depletion of myeloid cells in vivo. |
| Reactivity | Mouse |
| Applications | IHC-Fr, FC, IF, IHC-P, WB |
| Working Dilutions | Flow Cytometry: 1:50 - 1:100, Use 10µl of the suggested working dilution to label 106 cells in 100µl. |
| Form | Liquid |
| Diluent Buffer | Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide. |
| Concentration | 1 mg/ml |
| Storage | Store undiluted at 4°C. Do not freeze! Should this product contain a precipitate we recommend microcentrifugation before use. |
| Supplier | Antibodies.com |
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