| Product Name | Anti-Mannose Receptor Antibody [MR5D3] |
|---|---|
| Description | Rat monoclonal [MR5D3] antibody to Mannose Receptor. |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | MR5D3 |
| Conjugate | Unconjugated |
| Immunogen | Chimaeric CRD4-7-Fc protein. |
| Isotype | IgG2a |
| Target | Mannose Receptor |
| Specificity | This antibody recognises the mouse mannose receptor, a ~175 kDa type 1 membrane glycoprotein that is also known as CD206. CD206 is expressed on most tissue macrophages, certain endothelial cells and in vitro derived dendritic cells. The mannose receptor, CD206, is composed of a N-terminal cysteine-rich domain, a fibronectin type II domain, eight tandemly arranged C-type lectin domains (CTLD), a transmembrane domain, and a cytoplasmic domain. The terminal cysteine-rich domain binds sulfated sugars, and the CTLD recognises carbohydrates terminating in mannose, fucose and N-acetylglucosamine, all sugars found on microorganisms and on some endogenous proteins. Rat anti mouse CD206 antibody, clone MR5D3 has been reported to be non-inhibitory for the binding of the mannose receptor to carbohydrate ligands. Clone MR5D3 has also been shown to work in western blotting (Martinez-Pomares et al. 2003 and Su et al. 2005). |
| Reactivity | Mouse |
| Applications | IHC-Fr, FC, IF, IP |
| Working Dilutions | FC 1: 1:10 - 1:20, Use 10µl of the suggested working dilution to label 106 cell in 100µl |
| Form | Liquid |
| Diluent Buffer | Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide. |
| Concentration | 1 mg/ml |
| Storage | Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. |
| Supplier | Antibodies.com |
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