| Product Name | Anti-Melanoma associated Antigen Monoclonal Antibody |
|---|---|
| Description | This gene encodes a transporter protein that mediates melanin synthesis. The protein is expressed in a high percentage of melanoma cell lines. Mutations in this gene are a cause of oculocutaneous albinism type 4, and polymorphisms in this gene are associated with variations in skin and hair color. Multiple transcript variants encoding different isoforms have been found for this gene. NKI/C-3 recognizes a melanoma-associated antigen present in the cytoplasm of melanoma cells and nevocellular nevi, in contrast to most mabs against melanoma-associated antigens present in the membrane, which are not retained after formalin fixation. Therefore, the mab is very useful for paraffin sections in routine pathology. Cross reactivity with carcinoids, medullary carcinomas of the thyroid, and occasionally prostatic, primary breast, avarian, and lung carcinomas may be observed. No reaction is seen with basal cell carcinomas or brain tumors. NKI/C-3 does not react with normal melanocytes or other normal tissues except for mastcells, histiocytes, salivary glands, bronchial glands, pancreatic und prostatic epithelium. For the diagnosis of melanoma, the antibody should be used in combination with other antibodies (e.g. cytokeratin immunoreactivity indicative for carcinoma versus vimentin immunoreactivity indicative for melanoma). |
| Synonyms | 1A1, AIM1, MATP, OCA4, SHEP5 |
| Clonality | Monoclonal |
| Clone | NK1/C3 |
| Immunogen | Human melanoma cells |
| Isotype | IgG1 |
| Specificity | In immunoblotting the antibody reacts with diffuse protein bands between 25 and 100 kD. All epithelium derived neoplasms |
| Reactivity | Human |
| Applications | ELISA, IHC, WB |
| Form | Stabilized ascites containing 0.1% sodium azide |
| Storage | 2-8C for immediate use, or at -2°C (aliquot) |
| References | Van Duinen, S.G. et al. (1984) Cancer 53: 1566-1573. |
| Background | This gene encodes a transporter protein that mediates melanin synthesis. The protein is expressed in a high percentage of melanoma cell lines. Mutations in this gene are a cause of oculocutaneous albinism type 4, and polymorphisms in this gene are associated with variations in skin and hair color. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Supplier | ARP |
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