Anti-Mouse IFN alpha In Vivo Antibody - Ultra Low Endotoxin (TIF-3C5)
| Product Name | Anti-Mouse IFN alpha In Vivo Antibody - Ultra Low Endotoxin (TIF-3C5) |
|---|---|
| Synonyms | IFNa, interferon alpha |
| Host | Hamster |
| Clonality | Monoclonal |
| Clone | TIF-3C5 |
| Isotype | Armenian Hamster IgG |
| Target | IFN alpha |
| Reactivity | Mouse |
| Applications | FA, WB |
| Diluent Buffer | 0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. |
| Reconstitution | Functional Assay: In vitro activity of TIF-3C5 - inhibits IFNalpha -induced (IFNalpha-A, -1, -4, -5, -11 and 13) Stat1 phosphorylation in vitro. [TIF-1D6 does not block in vitro functional activity]. - blocks IFNA (IFNA-A, -1, -4, -5, -11 and 13) induction of MHC-I expression (H2-Kb) using L929 or fibrosarcoma cell lines in a dose dependent manner. - neutralizes IFNA-induced antiviral activity in vitro following infection of L929 cells with VSV. Functional activity of both recombinant (IFNalpha-A, -1, -4, -5, -11 and 13) and natural IFNalpha can be blocked with no neutralization of IFNgor IFNb. In vivo activity of TIF-3C5 - TIF-3C5 circulates with a half-life of 4 days - blockade of IFNa by TIF-3C5 increases the lethality of mice infected with West Nile Virus (WNV), similar to susceptibility of Irf7-/- mice. Each investigator should determine their own optimal working dilution for specific applications. |
| Concentration | ≥ 2.0 mg/ml |
| Purity | >98% by SDS-PAGE and HPLC |
| Uniprot | P01572 |
| Storage | anti-IFN alpha In Vivo Antibody - Ultra Low Endotoxin (TIF-3C5) is stable when stored at 2-8°C. For long-term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles. |
| Notes | Products are for research use only. Not for use in diagnostic or therapeutic procedures. |
| Supplier | Ichorbio |
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