Anti-Mouse PD-1 In Vivo Antibody - Ultra Low Endotoxin (29F.1A12)
| Product Name | Anti-Mouse PD-1 In Vivo Antibody - Ultra Low Endotoxin (29F.1A12) |
|---|---|
| Synonyms | Programmed cell death protein 1, Pdcd1, CD279 |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | 29F.1A12 |
| Immunogen | PD-1 cDNA followed by PD-1-Ig fusion protein |
| Isotype | Rat IgG2a κ |
| Target | PD-1 |
| Specificity | Anti-PD-1 In Vivo Antibody - Ultra Low Endotoxin (29F.1A12) recognizes an epitope on Mouse PD-1. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE. |
| Reactivity | Mouse |
| Applications | IHC-Fr, Blocking, FC, WB |
| Diluent Buffer | 0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added. |
| Reconstitution | Western Blotting: To detect Mouse PD-1 this antibody can be used at a concentration of 30 µg/ml. Blocking: The 29F.1A12 antibody has been shown to block the binding of PD-1 to its ligands in vivo. For immune checkpoint antibodies 200 ug per mouse per injection is the standard dosage in the literature. NOTE: The response is model dependent. We have seen some tumor lines in mice that grow progressively. If we treat the animal with anti-PD-1 (days 3,6,9 @ 200 ug per animal per injection) then the tumor will regress, and disappear. Each investigator should determine their own optimal working dilution for specific applications. |
| Concentration | ≥ 2.0 mg/ml |
| Purity | >98% by SDS-PAGE and HPLC |
| Uniprot | Q02242 |
| Storage | anti-PD-1 In Vivo Antibody - Ultra Low Endotoxin (29F.1A12) is stable when stored at 2-8°C for at least four (4) weeks. For long-term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles. |
| Notes | Products are for research use only. Not for use in diagnostic or therapeutic procedures. |
| Supplier | Ichorbio |
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