| Product Name | Anti-N antigen Monoclonal Antibody, PE |
|---|---|
| Description | Mouse monoclonal antibody to human N antigen-PE conjugate. The blood group N antigen is expressed on the erythrocyte sialoglycoproteins Glycophorin A (GPA, where it is polymorphic, CD 235a) and Glycophorin B (GPB, CD 235b). The complete amino acid sequence of both of these highly homologous membrane glycoproteins is known. The nature of the amino acids at positions 1 and 5 (leucine and glutamic acid respectively) defines the N antigen on both GPA and GPB. Glycophorins A and B are heavily glycosylated with numerous O- glycans containing sialic acid. The reactivity of some anti-N reagents is affected by the degree of sialylation of the antigen. The antigen is found on erythroid cells and K562 and HEL erythroleukaemia cell lines. All normal erythrocytes express N antigen on GPB: MN and NN erythrocytes also express N antigen on GPA. There are approximately 2-10 x 105 GPA and 8-25 x 104 GPB molecules per erythrocyte. Rare individuals lacking GPA and GPB are known. |
| Synonyms | GPA, Glycophorin A, CD235a, Glycophorin B, GPB, CD235b, NN, MN |
| Clonality | Monoclonal |
| Clone | BRIC 157 |
| Immunogen | Found on erythroid cells, K562 and the HEL erythroleukemia cell lines. All normal erythrocytes express N antigen on GPB: MN and NN erythrocytes also express N antigen on GPA. |
| Isotype | Mouse IgG1 kappa light chain |
| Reactivity | Human |
| Applications | FC |
| Form | PE-conjugate, in PBS buffer, pH 7.2 containing 0.1% sodium azide, 1% BSA |
| Storage | 2-8C, do not freeze |
| References | 1. Reid ME, Lomas-Francis C. (1997) The blood group antigen facts book. Academic Press, London 2. Anstee, D.J., (1990) Vox Sang; 58: 1 20 (Review). 3. Langlois R.G. et al.,(1990) Cytometry 11: 513-521 |
| Supplier | ARP |
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