Anti-NCK1 (clone AT6B3)

Catalog
01-A0274
$0.00
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Antibodies, Monoclonal Antibodies
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Product Name Anti-NCK1 (clone AT6B3)
Description NCK1 (NCK adaptor protein 1) is a 377 amino acid protein. NCK1 belongs to the adaptor family of proteins. It is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. NCK1 is located in the cytoplasm, but shuttles between the cytoplasm and the nucleus. Overexpression of Nck1 in NIH/3T3 cells decreases DNA synthesis stimulated by EGF.
Reactivity Human
Applications Dot blot, ELISA, WB
Clone Anti-human NCK1 mAb, clone AT6B3, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human NCK1 protein.
Concentration 0.1mg/ml
Form Liquid. Supplied in Phosphate-Buffered Saline (pH 7.4) containing 10% Glycerol, 0.02% Sodium Azide.
Species Mouse
Host Mouse
Isotype K, IgG3
Immunogen Recombinant human NCK1 (1-377aa) purified from E. coli
NCBI Number NP_006144
References Braverman. L.E. and Quilliam. L.A. (1999) J Biol Chem 274: 5542-5549 Latreille. M. and Larose. L. (2006) J Biol Chem 281: 26633-26644 Kremer. B.E., et al. (2007) Cell 130: 837-850
Storage Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.
Synonyms NCK, NCKalpha, NCK adaptor protein 1 GRB4, melanoma NCK protein, MGC12668, NCK adaptor protein 2, NCK tyrosine kinase, NCKbeta, non catalytic region of tyrosine kinase, noncatalytic region of tyrosine kinase, beta, SH2/SH3 adaptor protein NCK alpha, SH2/SH3 adaptor protein NCK beta.
Working Dilutions Optimal dilution should be performed by user.

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