| Product Name | Anti-Nucleolar Protein NO38 Monoclonal Antibody |
|---|---|
| Description | Nucleophosmin (NPM), also known as nucleolar phosphoprotein B23 or numatrin, is a protein that in humans is encoded by the NPM1 gene. NPM1 is associated with nucleolar ribonucleoprotein structures and bind single-stranded and double-stranded nucleic acids, but it binds preferentially G-Quadruplex forming nucleic acids. It is involved in the biogenesis of ribosomes and may assist small basic proteins in their transport to the nucleolus. Its regulation through SUMOylation (by SENP3 and SENP5) is another facet of the proteins's regulation and cellular functions. It is located in the nucleolus, but it can be translocated to the nucleoplasm in case of serum starvation or treatment with anticancer drugs. Mouse Monoclonal Antibody to Nucleolar Protein NO38. |
| Clonality | Monoclonal |
| Clone | NO-63 |
| Immunogen | Nucleolar fraction prepared from Xenopus laevis oocytes |
| Isotype | IgG2a |
| Specificity | The antibody recognizes a 38 kD nucleolar protein (NO38) in Xenopus (this protein has also been termed B23, nucleophosmin, numatrin in other species). NO63 stains specifically the granular component of the nucleolus ans small nucleolar dots in interphase cells. During mitosis the protein is localized accumulated around the condensed chromosomes. |
| Reactivity | Amphibian, Gallus |
| Applications | IP, WB |
| Form | Ascites containing 0.05% NaN3 |
| Storage | 2-8C for immediate use, or at -2°C (aliquot) |
| References | Schmidt-Zachmann, MS et al. (1987) EMBO J. 6: 1881-1890Zirwes, RF. et al. (1997) Mol. Biol. Cell 8: 231-248Zirwes, RF. et al. (1997) PNAS USA 94: 11387-11392 |
| Background | Nucleophosmin (NPM), also known as nucleolar phosphoprotein B23 or numatrin, is a protein that in humans is encoded by the NPM1 gene. NPM1 is associated with nucleolar ribonucleoprotein structures and bind single-stranded and double-stranded nucleic acids, but it binds preferentially G-Quadruplex forming nucleic acids. It is involved in the biogenesis of ribosomes and may assist small basic proteins in their transport to the nucleolus. Its regulation through SUMOylation (by SENP3 and SENP5) is another facet of the proteins's regulation and cellular functions. It is located in the nucleolus, but it can be translocated to the nucleoplasm in case of serum starvation or treatment with anticancer drugs. |
| Supplier | ARP |
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