| Product Name | Anti-p21/WAF-1 Monoclonal Antibody |
|---|---|
| Description | This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Multiple alternatively spliced variants have been found for this gene. p21 is a nuclear 21 kD protein, a product of the WAF-1/CIP1 gene. It is a constituent of a large complex of nuclear proteins, including cyclins, cyclin dependent kinases and PCNA. After activation by the wild type tumor suppressor protein p53, WAF-1 acts as an inhibitor of the cell cycle during G1 phase. Normal cells generally display a rather intense nuclear p21expression. In several malignancies (e.g. breast cancer, bladder cancer) it has been shown that the presence of mutations in the p53 gene are of prognostic significance. Demonstration of low expression of p21 in a p53 positive tumor will increase the likelihood of the presence of a p53 mutation. P21 immunostaining is therefore most often used in combination with p53 to confirm the presence of a p53 mutation. Overexpression of p53 with normal intensity of p21 argues against a p53 mutation, while p53 overexpression in combination of low or absent p21 expression is consistent with a p53 mutation. |
| Synonyms | P21, CIP1, SDI1, WAF1, CAP20, CDKN1, MDA-6, p21CIP1 |
| Clonality | Monoclonal |
| Clone | WA-1 |
| Immunogen | Recombinant p21/WAF-1 protein |
| Isotype | IgG1 |
| Specificity | p21 immunostaining is most often used in combination with p53 to confirm the presence of a p53 mutation. p53 overexpression in combination with low or absent p21 expression is consistent with a p53 mutation. |
| Reactivity | Mammal |
| Applications | IHC, WB |
| Form | Hybridoma culture Supernatant |
| Storage | 2-8C |
| Background | This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Multiple alternatively spliced variants have been found for this gene. |
| Supplier | ARP |
All Research Products are sold for laboratory RESEARCH USE ONLY and ARE NOT TO BE USED FOR HUMAN OR ANIMAL THERAPEUTIC OR DIAGNOSTIC APPLICATIONS. The information presented is believed to be accurate; however, said information and products are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. Nothing disclosed herein is to be construed as a recommendation to use our products in violation of any patents. ARP American Research Products, Inc. does not submit its products for regulatory review by any government body or other organization, and we do not validate them for clinical, therapeutic or diagnostic use, or for safety and effectiveness. You are solely responsible for making sure that the way you use the products complies with applicable laws, regulations and governmental policies and for obtaining all necessary approvals, intellectual property rights, licenses and permissions that you may need related to your use. Under no circumstances shall ARP American Research Products, Inc. be liable for damages, whether consequential, compensatory, incidental or special, strict liability or negligence, breach of warranty or any other theory arising out of the use of the products available from ARP American Research Products, Inc. Nothing contained herein warrants that the use of the products will not infringe on the claims of any patents covering the product itself or the use thereof in combination with other products or in the operation of any process. ARP American Research Products, Inc. disclaims any and all representations or warranties of any kind whatsoever, express or implied, including without limitation any implied warranties of merchantability or fitness for a particular purpose, of non-infringement, or regarding results obtained through the use of any product, whether arising from a statute or otherwise in law or from a course of performance, dealing or usage of trade.
