| Product Name | Anti-RNA Polymerase II Monoclonal Antibody |
|---|---|
| Description | RNA polymerase II (also called RNAP II and Pol II) is an enzyme found in eukaryotic cells. It catalyzes the transcription of DNA to synthesize precursors of mRNA and most snRNA and microRNA.[2][3] A 550 kDa complex of 12 subunits, RNAP II is the most studied type of RNA polymerase. A wide range of transcription factors are required for it to bind to upstream gene promoters and begin transcription. Mouse Monoclonal Antibody to RNA Polymerase II. Reactive polypeptides after SDS-PAGE: 200 kD band of nonphosphorylated and 240 kD band of phosphorylated Pol IIA. Epitope recognized: 794DDYGPESRGFVENSYLA822 |
| Synonyms | RNAP |
| Clonality | Monoclonal |
| Clone | ARNA-3 |
| Immunogen | Purified RNA Polymerase II |
| Isotype | IgG1 |
| Specificity | ARNA-3 is excellent for detection of transcription activity, e.g. puffs in polytene chromosomes. Reacts with RNA polymerase II (Mr 175,000 subunit of RNAP II) in biological materials. |
| Reactivity | Human, Insect |
| Applications | IHC, WB |
| Form | Hybridoma culture supernatant |
| Storage | 2-8C |
| References | Kramer, A. et al. (1980) Molec. Gen. Genet. 180: 193-199Petersen, G. et al. (1995) Molec. Gen. Genet. 249: 425-431Kontermann RE et al. (1995) Biol Chem Hoppe Seyler 376: 473-481Nayler O et al. (1998) Nucl Acids Res 26: 3542-3549Dubois M-F et al. (1999) Nucleic Acids Re 27: 1338-1344 |
| Background | RNA polymerase II (also called RNAP II and Pol II) is an enzyme found in eukaryotic cells. It catalyzes the transcription of DNA to synthesize precursors of mRNA and most snRNA and microRNA.[2][3] A 550 kDa complex of 12 subunits, RNAP II is the most studied type of RNA polymerase. A wide range of transcription factors are required for it to bind to upstream gene promoters and begin transcription. |
| Supplier | ARP |
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