| Product Name | Anti-Synaptopodin (INN) Polyclonal Antibody |
|---|---|
| Description | The antibody reacts specifically with an epitope in the internal part of synaptopodin, a prolin-rich actin-binding protein with 2 binding sites for actin. Synaptopodin belongs to actin-binding pro-teins, it has first been localized in podocytes and a subset of telencephalic postsynaptic densities. In human tissue synaptopodin has a molecular weight of 73.7 kD and pI of 9.38 (calculated from sequence data); in mouse the corre-sponding data are 74 kD, pI 9.27. In SDS-PAGE the antigen appears as 100 kD polypeptide in brain and 110 kD polypeptide in kidney (attributed to posttransla-tional modifications). In Western blot analysis the antibody also reacts with a 44 kD degradation fragment of synaptopodin. |
| Synonyms | SYNPO |
| Clonality | Polyclonal |
| Immunogen | Synthetic peptides (mouse internal N-terminal sequence), coupled to KLH |
| Specificity | The antibody recognizes differentiated podocytes (glomerular visceral epithelial cells) in vivo and in vitro (weaker additional reaction with arterial endothelial cells), co-localization with a-actinin. 03-GP94-INN reacts with a subset of exclusively telencephalic synapses. Differentiation-dependent expression during postnatal maturation of murine brain. Differentiation-dependent expression in cultured hippocampal neurons. |
| Reactivity | Human, Mouse |
| Applications | IHC, WB |
| Form | Stabilized Serum (Synpo-mINN) |
| Storage | 2-8C |
| References | Mundel P, Gilbert P, and W Kriz. Podocytes in Glomerulus of Rat Kidney Express a Characteristic 44 kD Protein. J Histochem and Cyto- chem Vol 39 No 8:1047-1056 (1991) Mundel P and W Kritz. Structure and function of podocytes: an update. Anat Embryol 192:385-397 (1995) Mundel P, Heid HW, Mundel TM, KrÌÄå_ger M, Reiser J, Kriz W. Synaptopodin, an actin-associated protein in telencephalic dendrites and in renal podocytes. J Cell Biol Vol 139 (1):193-204 (1997) Asanuma K, Kim K, Oh J, Giardino L, Chabanis S, Faul C, Reiser J, Mundel P: synaptopodin regulates the actin-bundling activity of alpha- actinin in an isoform-specific manner. J Clin Invest 115, 1188-1198 (2005) Zhang XL, PÌÄå¦schel B, Faul C, Upreti C, Stanton PK, Mundel P: Essential role for synaptopodin in dendritic spine plasticity of the developing hippocampus. J Neurosci. 2013 Jul 24;33(30):12510-8. doi: 10.1523/JNEUROSCI.2983-12.2013 |
| Background | The antibody reacts specifically with an epitope in the internal part of synaptopodin, a prolin-rich actin-binding protein with 2 binding sites for actin. Synaptopodin belongs to actin-binding pro-teins, it has first been localized in podocytes and a subset of telencephalic postsynaptic densities. In human tissue synaptopodin has a molecular weight of 73.7 kD and pI of 9.38 (calculated from sequence data); in mouse the corre-sponding data are 74 kD, pI 9.27. In SDS-PAGE the antigen appears as 100 kD polypeptide in brain and 110 kD polypeptide in kidney (attributed to posttransla-tional modifications). In Western blot analysis the antibody also reacts with a 44 kD degradation fragment of synaptopodin. |
| Supplier | ARP |
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