| Product Name | Anti-TIM 1 Antibody [RMT1-10] |
|---|---|
| Description | Rat monoclonal [RMT1-10] antibody to TIM 1. |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | RMT1-10 |
| Conjugate | Unconjugated |
| Immunogen | Full length mouse Tim-1-Ig, containing both the IgV and mucin domains of Tim-1. |
| Isotype | IgG2a |
| Target | TIM 1 |
| Specificity | This antibody recognises mouse Tim-1 (T-cell immunoglobulin mucin 1), a cell surface glycoprotein first identified as the hepatitis A virus cellular receptor (HAVCR). TIM-1 is highly expressed in the liver (isoform Tim-1a) and the kidneys (isoform Tim-1b).Tim-1 is highly expressed by activated CD4+ T cells, acting as a positive/negative co-stimulatory molecule of T cell proliferation, cytokine production and tolerance abrogation, and is the receptor for Tim-4, expressed on APCs. Tim-1 expression is greater on Th2 than Th1 cells, interaction between Tim-1 on Th2 cells and Tim-4 on dendritic cells (DCs), enhances Th2 cell function, and have implicated Tim-1 as a critical player in the development of atopic disease, and in particular airway hypersensitivity.In humans, polymorphic forms of Tim-1 are associated with increased susceptibility to asthma, eczema and rheumatoid arthritis. Rat anti Mouse TIM-1 antibody, clone RMT1-10 promotes Th2 responses, and inhibits antigen-specific T cell proliferation, in contrast to the agonistic function of many Tim-1 antibodies. Rat anti Mouse TIM-1 antibody, clone RMT1-10 reduces the severity of experimental autoimmune encephalomyelitis and delays disease onset in mice. |
| Reactivity | Mouse |
| Applications | ELISA, FC, IF |
| Working Dilutions | Flow Cytometry: 1:25 - 1:200, Use 10µl of the suggested working dilution to label 106 cells in 100µl. |
| Form | Liquid |
| Diluent Buffer | Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide. |
| Concentration | 1 mg/ml |
| Storage | Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. |
| Supplier | Antibodies.com |
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