| Product Name | Anti-TIM 4 Antibody [RMT4-54] |
|---|---|
| Description | Rat monoclonal [RMT4-54] antibody to TIM 4. |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | RMT4-54 |
| Conjugate | Unconjugated |
| Immunogen | Tim-4-Ig, consisting of amino acids 1-288 of mouse Tim-4, coupled with the Fc portion of mouse IgG2a. |
| Isotype | IgG2a |
| Target | TIM 4 |
| Amino Acid Sequence | MSKGLLLLWLVTELWWLYLTPAASEDTIIGFLGQPVTLPCHYLSWSQSRNSMCWGKGSCPNSKCNAELLRTDGTRIISRKSTKYTLLGKVQFGEVSLTISNTNRGDSGVYCCRIEVPGWFNDVKKNVRLELRRATTTKKPTTTTRPTTTPYVTTTTPELLPTTVMTTSVLPTTTPPQTLATTAFSTAVTTCPSTTPGSFSQETTKGSAFTTESETLPASNHSQRSMMTISTDIAVLRPTGSNPGILPSTSQLTTQKTTLTTSESLQKTTKSHQINSRQTILIIACCVG |
| Protein Length | 288 |
| Specificity | This antibody recognises mouse Tim-4 (T-cell immunoglobulin mucin 4), a transmembrane glycoprotein and member of the immunoglobulin superfamily, exclusively expressed by antigen-presenting cells, which acts as the natural ligand of Tim-1, involved in T-cell expansion and cytokine production.Tim-4 is necessary for the phagocytosis of apoptotic cells by peritoneal macrophages and interaction between Tim-1 on Th2 cells and Tim-4 on DCs, enhances Th2 cell function. The TIM-4 gene locus is associated with predisposition to asthma in both mice and humans and may be considered as a candidate disease gene for asthma.Rat anti Mouse TIM-4 antibody, clone RMT4-54 inhibits the efficient in vitro phagocytosis of apoptotic cells by resident peritoneal Mac1+ cells), but not inflammatory peritoneal exudate Mac1+ cells. |
| Reactivity | Mouse |
| Applications | FC, IF, IHC-P |
| Working Dilutions | Flow Cytometry: 1:25 - 1:200, Use 10µl of the suggested working dilution to label 106 cells in 100µl. |
| Form | Liquid |
| Diluent Buffer | Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide. |
| Concentration | 1 mg/ml |
| Storage | Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. |
| Supplier | Antibodies.com |
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