| Product Name | Anti-Vinculin Antibody [VCL/3617] |
|---|---|
| Description | Mouse monoclonal [VCL/3617] antibody to Vinculin. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | VCL/3617 |
| Conjugate | Unconjugated |
| Immunogen | Recombinant full-length human Vinculin protein. |
| Isotype | IgG1 |
| Molecular Weight | 125-135 kDa |
| Target | Vinculin |
| Specificity | This antibody recognizes both Vinculin (125kDa) and meta-Vinculin (135kDa). Focal adhesions are identified as areas within the plasma membrane of tissue culture cells that adhere tightly to the underlying substrate. In vivo, these regions are involved in the adhesion of cells to the extracellular matrix. Paxillin and vinculin are cytoskeletal, focal adhesion proteins that are components of a protein complex which links the Actin network to the plasma membrane. Vinculin binding sites have been identified on other cytoskeletal proteins, including Talin and -Actinin has been shown to link Actin to integrins in the plasma membrane through interactions with the vinculin and Talin complex or by a direct interaction with integrin. |
| Reactivity | Aves, Frog, Bovine, Fish, Human, Mouse, Porcine, Rabbit, Rat |
| Applications | FC, IF, IHC-P |
| Working Dilutions | IF: 1-2 µg/ml, Flow Cytometry: 1-2 µg/million cells, IHC-P: 1-2 µg/ml |
| Form | Liquid |
| Diluent Buffer | Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide. |
| Concentration | 200 µg/ml |
| Storage | Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. |
| Supplier | Antibodies.com |
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