ANXA1 ELISA kit (Human)

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SEE787Hu
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Product Name ANXA1 ELISA kit (Human)
Description This assay has high sensitivity and excellent specificity for detection of Annexin A1 (ANXA1). No significant cross-reactivity or interference between Annexin A1 (ANXA1) and analogues was observed.
Synonyms ANX-A1; ANX1; LPC1; Lipocortin I; Chromobindin-9; Calpactin II; Phospholipase A2 inhibitory protein
Method Sandwich
Detection Range 78-5,000pg/mL
Sensitivity 32pg/mL
Target ANXA1
Specificity This assay has high sensitivity and excellent specificity for detection of Annexin A1 (ANXA1). No significant cross-reactivity or interference between Annexin A1 (ANXA1) and analogues was observed.
Reactivity Human
Sample Types Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids
Reconstitution 1.Prepare all reagents, samples and standards; 2.Add 100uL standard or sample to each well.Incubate 2 hours at 37℃; 3.Aspirate and add 100uL prepared Detection Reagent A.Incubate 1 hour at 37℃; 4.Aspirate and wash 3 times; 5.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 6.Aspirate and wash 5 times; 7.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 8.Add 50uL Stop Solution.Read at 450nm immediately.
Uniprot P04083
Storage 4°C for 1 month/ -20°C for long-term(within shelf life)
Notes Colormetric
References Dysregulation of Anti-Inflammatory Annexin A1 Expression in Progressive Crohns Disease;Modulatory Effect of Phytoestrogens and Curcumin on Induction of Annexin 1 in Human Peripheral Blood Mononuclear Cells and their Inhibitory Effect on Secretory Phospholipase A2;Characterization of secretomes from a human blood brain barrier endothelial cells in-vitro model after ischemia by stable isotope labeling with aminoacids in cell culture (SILAC);Annexin A1 Is Increased in the Plasma of Preeclamptic Women;A1 CA19-9 ;Mesenchymal stromal cells improve human islet function through released products and extracellular matrix.;Proresolving protein Annexin A1: The role in type 2 diabetes mellitus and obesity;Annexin A1 is elevated in patients with COPD and affects lung fibroblast function;Applying host disease status biomarkers to therapeutic response monitoring in invasive aspergillosis patients;EFFECTS OF MULTICOMPONENT EXERCISE ON PROGRESSION OF BRAIN ATROPHY AND COGNITION IN AMNESTIC MILD COGNITIVE IMPAIRMENT …;Resolution of inflammation in neuromyelitis optica spectrum disorders;Annexin A1 accounts for an anti-inflammatory binding target of sesamin metabolites;Expression of annexin-A1 in blood and tissue leukocytes of leprosy patients;
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