ANXA2 ELISA kit (Human)

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SEB944Hu
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Product Name ANXA2 ELISA kit (Human)
Description This assay has high sensitivity and excellent specificity for detection of Annexin A2 (ANXA2). No significant cross-reactivity or interference between Annexin A2 (ANXA2) and analogues was observed.
Synonyms ANX-A2; ANX2L4; CAL1H; LIP2; LPC2; LPC2D; P36; PAP-IV; Protein I; Chromobindin-8; Calpactin-1 heavy chain; Lipocortin II; Placental anticoagulant protein IV
Method Sandwich
Detection Range 0.156-10ng/mL
Sensitivity 0.058ng/mL
Target ANXA2
Specificity This assay has high sensitivity and excellent specificity for detection of Annexin A2 (ANXA2). No significant cross-reactivity or interference between Annexin A2 (ANXA2) and analogues was observed.
Reactivity Human
Sample Types Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids
Reconstitution 1.Prepare all reagents, samples and standards; 2.Add 100uL standard or sample to each well.Incubate 2 hours at 37℃; 3.Aspirate and add 100uL prepared Detection Reagent A.Incubate 1 hour at 37℃; 4.Aspirate and wash 3 times; 5.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 6.Aspirate and wash 5 times; 7.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 8.Add 50uL Stop Solution.Read at 450nm immediately.
Uniprot P07355
Storage 4°C for 1 month/ -20°C for long-term(within shelf life)
Notes Colormetric
References Subcellular proteome analysis unraveled annexin A2 related to immune liver fibrosis;Expression characteristics and diagnostic value of annexin A2 in hepatocellular carcinoma;Annexin A2 is not a good biomarker for hepatocellular carcinoma in cirrhosis;Annexin A2 Versus Afp as An Efficient Diagnostic Serum Marker for Hepatocellular Carcinoma;Serum annexin A2 levels in acute brucellosis and brucellar spondylodiscitis;Circulating annexin A2 as a biomarker in gastric cancer patients: Correlation with clinical variables;Serum Levels of Annexin A2 as a Candidate Biomarker for Hepatic Fibrosis in Patients With Chronic Hepatitis B;Up-regulation of annexin A2 expression predicates advanced clinicopathological features and poor prognosis in hepatocellular carcinoma;Circulating;Evaluation of annexin A2 and as potential biomarkers for hepatocellular carcinoma;Stairs instead of elevators at the workplace decreases PCSK9 levels in a healthy population;Serum Annexin A2 Level Is Associated With Diagnosis and Prognosis in Patients With OralSquamous Cell Carcinoma.;Assessment of annexin A5 and annexin A2 levels as biomarkers for pre-eclampsia: A pilot study.;Epigallocatechin gallate induces an up-regulation of LDL receptor accompanied by a reduction of PCSK9 via the annexin A2-independent pathway in HepG2 cells.;Expression of annexin A2 in adenomyosis and dysmenorrhea;Extracellular Vesicles-Based Biomarkers Represent a Promising Liquid Biopsy in Endometrial Cancer;PLASMA BIOMARKER FOR OVARIAN CANCER;Diagnostic Value of Plasma Annexin A2 in Early-Stage High-Grade Serous Ovarian Cancer. Diagnostics 2021, 11, 69;Search for Effective Serum Tumor Markers for Early Diagnosis of Hepatocellular Carcinoma Associated with Hepatitis C;
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