AP13 ELISA kit (Human)

Category: Assay Kits
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CEB887Hu
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Product Name AP13 ELISA kit (Human)
Description This assay has high sensitivity and excellent specificity for detection of Apelin 13 (AP13). No significant cross-reactivity or interference between Apelin 13 (AP13) and analogues was observed.
Synonyms APLN13
Method Competitive
Detection Range 12.35-1,000pg/mL
Sensitivity 4.83pg/mL
Target AP13
Specificity This assay has high sensitivity and excellent specificity for detection of Apelin 13 (AP13). No significant cross-reactivity or interference between Apelin 13 (AP13) and analogues was observed.
Reactivity Human
Sample Types Serum, Plasma And Other Biological Fluids
Reconstitution 1.Prepare all reagents, samples and standards; 2.Add 50uL standard or sample to each well. And then add 50uL prepared Detection Reagent A immediately. Shake and mix.Incubate 1 hour at 37℃; 3.Aspirate and wash 3 times; 4.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 5.Aspirate and wash 5 times; 6.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 7.Add 50uL Stop Solution.Read at 450 nm immediately.
Storage 4°C for 1 month/ -20°C for long-term(within shelf life)
Notes Colormetric
References Apelin in Plasma and Vitreous and in Fibrovascular Retinal Membranes of Patients with Proliferative Diabetic Retinopathy;Effect of Ramadan fasting on serum concentration of apelin-13 and new obesity indices in healthy adult men;The Role of Apelin 13 in Progression of Chronic Kidney Disease.;Role of Circulating Serum Apelin-13 Levels in Glomerulonephritis: A Pilot Study;Biochemical and genetic role of apelin in essential hypertension and acute coronarysyndrome.;Serum concentrations of apelin-17 isoform vary in accordance to blood pressure categories in individuals with obesity class 3;Effect of fermented food consumption on biochemical parameters and adipokines levels;Impact of apelin-13 on the development of coronary artery ectasia;
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