APOA1 ELISA kit (Mouse)

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SEA519Mu
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Product Name APOA1 ELISA kit (Mouse)
Description This assay has high sensitivity and excellent specificity for detection of Apolipoprotein A1 (APOA1). No significant cross-reactivity or interference between Apolipoprotein A1 (APOA1) and analogues was observed.
Synonyms Apo-A1; ApoA-1 Milano; ProapoA-I; Proapolipoprotein A-I; Truncated apolipoprotein A-I
Method Sandwich
Detection Range 1.56-100ng/mL
Sensitivity 0.69ng/mL
Target APOA1
Specificity This assay has high sensitivity and excellent specificity for detection of Apolipoprotein A1 (APOA1). No significant cross-reactivity or interference between Apolipoprotein A1 (APOA1) and analogues was observed.
Reactivity Mouse
Sample Types Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids
Reconstitution 1.Prepare all reagents, samples and standards; 2.Add 100uL standard or sample to each well.Incubate 2 hours at 37℃; 3.Aspirate and add 100uL prepared Detection Reagent A.Incubate 1 hour at 37℃; 4.Aspirate and wash 3 times; 5.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 6.Aspirate and wash 5 times; 7.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 8.Add 50uL Stop Solution.Read at 450nm immediately.
Uniprot Q00623
Storage 4°C for 1 month/ -20°C for long-term(within shelf life)
Notes Colormetric
References Hepatic lipase- and endothelial lipase-deficiency in mice promotes macrophage-to-feces RCT and HDL antioxidant properties;MicroRNA-33 Deficiency Reduces the Progression of Atherosclerotic Plaque in ApoE?/? Mice;DYRK1A overexpression decreases plasma lecithin: cholesterol acyltransferase activity and apolipoprotein AI levels;Deficiency of Clusterin Exacerbates High-Fat Diet-Induced Insulin Resistance in Male Mice ;Corrective effects of hepatotoxicity by hepatic Dyrk1a gene delivery in mice with intermediate hyperhomocysteinemia;Efficacy of tomato concentrates in mouse models of dyslipidemia and cancer;Apolipoprotein A-I inhibits experimental colitis and colitis-propelled carcinogenesis;Apolipoprotein AI inhibits experimental colitis and colitis-propelled carcinogenesis;Serum levels of 9α,11β-PGF2 and apolipoprotein A1 achieve high predictive power as biomarkers of anaphylaxis.;High-Density Lipoproteins Exert Pro-inflammatory Effects on Macrophages via Passive Cholesterol Depletion and PKC-NF-kB/STAT1-IRF1 Signaling;Effect of Ginkgo biloba Extract (EGb 761) on Serum Cholesterol Levels in Wild-type C57Bl/6 Mice;Systemic Alterations of Immune Response-Related Proteins during Glaucoma Development in the Murine Model DBA/2J;GP73 is a TBC-domain Rab GTPase-activating protein contributing to the pathogenesis of non-alcoholic fatty liver disease without obesity;
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