| Product Name | ApoB-100, Human, ELISA Kit |
|---|---|
| Description | Though the lipids (such as fat and cholesterol) are not soluble in water, apolipoproteins are carrier proteins that combine with lipids to form lipoprotein particles which are water-soluble and can be carried through water-based circulation (i.e., blood, lymph). Apolipoproteins are classified according to their forms and functions, and there are six major classes and several sub-classes. ApoB (ApolipoproteinB) is the primary apolipoprotein of LDL and is considered to reflect change of LDL level in blood. There are two types apolipoprotein in ApoB. One is ApoB-100 which correlates with cholesterol level well and is released as LDL and VLDL. Another is ApoB-48 which presents being incorporated into chylomicron sand correlates well with tryglyceride. Generally, the concentration of ApoB in blood has been measured as the total ofApoB-100 and ApoB-48. This kit can measure only ApoB-100 in plasma samples using ApoB-100 specific antibodies which don't recognize ApoB-48. This highly sensitive sandwich ELISA can also measure ApoB-100 in cell culture media or various fractionated lipoprotein samples. This kit is a solid phase sandwich ELISA using 2 kinds of highly specific antibodies. Tetra Methyl Benzidine (TMB) is used as a coloring agent (Chromogen). The strength of coloring is proportional to the quantities of Human ApoB-100. |
| Reactivity | Human |
| Background | Though the lipids (such as fat and cholesterol) are not soluble in water, apolipoproteins are carrier proteins that combine with lipids to form lipoprotein particles which are water-soluble and can be carried through water-based circulation (i.e., blood, lymph). Apolipoproteins are classified according to their forms and functions, and there are six major classes and several sub-classes. ApoB (ApolipoproteinB) is the primary apolipoprotein of LDL and is considered to reflect change of LDL level in blood. There are two types apolipoprotein in ApoB. One is ApoB-100 which correlates with cholesterol level well and is released as LDL and VLDL. Another is ApoB-48 which presents being incorporated into chylomicron sand correlates well with tryglyceride. Generally, the concentration of ApoB in blood has been measured as the total ofApoB-100 and ApoB-48. This kit can measure only ApoB-100 in plasma samples using ApoB-100 specific antibodies which don't recognize ApoB-48. This highly sensitive sandwich ELISA can also measure ApoB-100 in cell culture media or various fractionated lipoprotein samples. |
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