| Product Name | APOE ELISA kit (Human) |
|---|---|
| Description | This assay has high sensitivity and excellent specificity for detection of Apolipoprotein E (APOE). No significant cross-reactivity or interference between Apolipoprotein E (APOE) and analogues was observed. |
| Synonyms | Apo-E; AD2; Apoprotein; Alzheimer Disease 2(E4-Associated,Late Onset |
| Method | Sandwich |
| Detection Range | 4.7-300ng/mL |
| Sensitivity | 1.9ng/mL |
| Target | APOE |
| Specificity | This assay has high sensitivity and excellent specificity for detection of Apolipoprotein E (APOE). No significant cross-reactivity or interference between Apolipoprotein E (APOE) and analogues was observed. |
| Reactivity | Human |
| Sample Types | Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids |
| Reconstitution | 1.Prepare all reagents, samples and standards; 2.Add 100uL standard or sample to each well.Incubate 2 hours at 37℃; 3.Aspirate and add 100uL prepared Detection Reagent A.Incubate 1 hour at 37℃; 4.Aspirate and wash 3 times; 5.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 6.Aspirate and wash 5 times; 7.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 8.Add 50uL Stop Solution.Read at 450nm immediately. |
| Uniprot | P02649 |
| Storage | 4°C for 1 month/ -20°C for long-term(within shelf life) |
| Notes | Colormetric |
| References | Cerebrospinal fluid apolipoprotein E concentration decreases after seizure;Expression and clinical significance of apolipoprotein E in pancreatic ductal adenocarcinoma;Adipocyte Atp-Binding Cassette G1 Promotes Triglyceride Storage, Fat Mass Growth And Human Obesity;Frequencies of apolipoprotein E alleles in depressed patients undergoing hemodialysis – a case-control study;Culturing of HepG2 cells with human serum improve their functionality and suitability in studies of lipid metabolism;Adaptive immunity against gut microbiota enhances apoE-mediated immune regulation andreduces atherosclerosis and western-diet-related inflammation.;Deletion of plasma Phospholipid Transfer Protein (PLTP) increases microglial phagocytosis and reduces cerebral amyloid-β deposition in the J20 mouse …;Comparative proteomics analysis of serum proteins in gestational diabetes during early and middle stages of pregnancy;The Significance of Apolipoprotein E Measurement in the Screening of Fetal Down Syndrome;Discovery and validation of FBLN1 and ANT3 as potential biomarkers for early detection of cervical cancer;Iridium nanoclusters as high sensitive-tunable elemental labels for immunoassays: Determination of IgE and APOE in aqueous humor by inductively coupled …;Gold nanoclusters as elemental label for the sequential quantification of apolipoprotein E and metallothionein 2A in individual human cells of the retinal …; |
| Supplier | Cloud Clone |
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