| Product Name | ARL9, 1-123aa, Human |
|---|---|
| Description | ARL9 (ADP-ribosylation factor-like protein 9) belongs to two novel subfamilies coding for small GTPases that we found to be highly conserved among eukaryotes. Recombinant human ARL9 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer. |
| Synonyms | ADP-ribosylation factor-like 9, ADP ribosylation factor like protein 9, ADP ribosylation factor like 9 |
| Host | E. coli |
| Molecular Weight | 16.9kDa (145aa), confirmed by MALDI-TOF |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MEFLEIGGSK PFRSYWEMYL SKGLLLIFVV DSADHSRLPE AKKYLHQLIA ANPVLPLVVF ANKQDLEAAY HITDIHEALA LSEVGNDRKM FLFGTYLTKN GSEIPSTMQD AKDLIAQLAA DVQ |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 10% glycerol, 100mM NaCl |
| Concentration | 1mg/ml (determined by Bradford assay) |
| Purity | > 90% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Louro R, et al. (2004) Biochem Biophys Res Commun. 316(3):618-27. Pasqualato S., et al (2002) EMBO Rep. 3(11):1035-41. |
| Background | ARL9 (ADP-ribosylation factor-like protein 9) belongs to two novel subfamilies coding for small GTPases that we found to be highly conserved among eukaryotes. Recombinant human ARL9 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer. |
| Supplier | ARP |
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