| Product Name | ASD ELISA kit (General species) |
|---|---|
| Description | This assay has high sensitivity and excellent specificity for detection of Androstenedione (ASD). No significant cross-reactivity or interference between Androstenedione (ASD) and analogues was observed. |
| Synonyms | 4-Androstenedione; 4-Androstene-3,17-Dione |
| Method | Competitive |
| Detection Range | 123.5-10,000pg/mL |
| Sensitivity | 44.2pg/mL |
| Target | ASD |
| Specificity | This assay has high sensitivity and excellent specificity for detection of Androstenedione (ASD). No significant cross-reactivity or interference between Androstenedione (ASD) and analogues was observed. |
| Sample Types | Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids |
| Reconstitution | 1.Prepare all reagents, samples and standards; 2.Add 50uL standard or sample to each well. And then add 50uL prepared Detection Reagent A immediately. Shake and mix.Incubate 1 hour at 37℃; 3.Aspirate and wash 3 times; 4.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 5.Aspirate and wash 5 times; 6.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 7.Add 50uL Stop Solution.Read at 450 nm immediately. |
| Storage | 4°C for 1 month/ -20°C for long-term(within shelf life) |
| Notes | Colormetric |
| References | Association between Polycystic Ovary Syndrome and Cavia (Guinea pig )t Microbiota;Molecular characterization of insulin resistance and glycolytic metabolism in the rat uterus;Metformin Ameliorates Uterine Defects in a Rat Model of Polycystic Ovary Syndrome.;Uterine progesterone signaling is a target for metformin therapy in polycystic ovary syndrome;Melatonin Stimulates STAR Expression and Progesterone Production via Activation of the PI3K/AKT Pathway in Bovine Theca Cells;Evidence that downregulation of Wilms' tumor 1 (WT1) is involved in cortical stromal cell differentiation into theca cells in adult bovine ovaries;Wilms' tumor (WT1)(+/-KTS) variants decreases the progesterone secretion of bovine ovarian theca cells;A study on steroidogenic elaborations of stroma and their regulation in response to ovarian hormones in goats;Extracellular Vesicles of Bovine Small Follicular Fluid Promote Ovarian Cortical Stromal Cell Proliferation and Steroidogenesis; |
| Supplier | Cloud Clone |
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