| Product Name | ASNA1, 1-348aa, Human |
|---|---|
| Description | ASNA1, also as known as ARSA and TRC40, belongs to the arsA ATPase family. ASNA1 is the human homolog of the bacterial arsA gene. In E.coli, ArsA ATPase is the catalytic component of a multisubunit oxyanion pump that is responsible for resistance to arsenicals and antimonials. ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recombinant human ASNA1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Synonyms | ATPase ASNA1, ARSA-I, ARSA1, ASNA-I, GET3, hASNA-I, TRC40 |
| Host | E. coli |
| Molecular Weight | 19.6 kDa (168aa) confirmed by MALDI-TOF |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MGSMAAGVAG WGVEAEEFED APDVEPLEPT LSNIIEQRSL KWIFVGGKGG VGKTTCSCSL AVQLSKGRES VLIISTDPAH NISDAFDQKF SKVPTKVKGY DNLFAMEIDP SLGVAELPDE FFEEDNMLSM GKKMMQEAMS AFPGIDEAMS YAEVMRLVKG MNFSVVVFDT APTGHTLRLL NFPTIVERGL GRLMQIKNQI SPFISQMCNM LGLGDMNADQ LASKLEETLP VIRSVSEQFK DPEQTTFICV CIAEFLSLYE TERLIQELAK CKIDTHNIIV NQLVFPDPEK PCKMCEARHK IQAKYLDQME DLYEDFHIVK LPLLPHEVRG ADKVNTFSAL LLEPYKPPSA Q |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 40% glycerol, 2mM DTT |
| Concentration | 0.25 mg/ml (determined by Bradford assay) |
| Purity | > 90% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Stefanovic S., et al. (2007) Cell 128:1147-1159 Favaloro V., et al. (2008) J. Cell Sci. 121:1832-1840 |
| Background | ASNA1, also as known as ARSA and TRC40, belongs to the arsA ATPase family. ASNA1 is the human homolog of the bacterial arsA gene. In E.coli, ArsA ATPase is the catalytic component of a multisubunit oxyanion pump that is responsible for resistance to arsenicals and antimonials. ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recombinant human ASNA1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Supplier | ARP |
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